PRP Centrifuge Machine – Its Principle, Procedure and uses


A PRP centrifuge machine (platelet-rich plasma centrifuge) is a laboratory apparatus that separates and concentrates platelets from a blood sample. Platelets are tiny disc-shaped cells that aid in blood clotting and wound healing. PRP has been employed in a wide range of medical and dental treatments, including aesthetic surgery, hair restoration, and injury and chronic condition treatment.

Also read: Centrifuge machine parts types application

The PRP centrifuge works by rapidly spinning a blood sample, causing the heavier components, such as platelets, to separate from the lighter components, such as red and white blood cells and plasma. Platelets that have been isolated can then be collected and used in a PRP treatment.

PRP centrifuges are available in a variety of sizes and styles, including desktop and benchtop units, and can be used in a variety of environments such as hospitals, clinics, and laboratories. Some PRP centrifuges are specifically intended for use with PRP kits, which contain all of the equipment and instructions needed to prepare PRP. Other PRP centrifuges are more general-purpose, capable of separating blood components for diagnostic purposes.

What is PRP?

Before getting into the physics of PRP centrifuge machines, it’s important to understand Platelet-Rich Plasma (PRP) and its therapeutic potential. PRP is a concentrated mixture of platelets, growth factors, and other bioactive proteins found in human blood. Because these substances are essential in the body’s natural healing processes, PRP is a valuable resource for medical purposes.

Platelets are well known for their role in blood clotting, although they have a greater significance. Platelets are rich in growth factors, which promote cell proliferation, tissue repair, and regeneration. This extraordinary quality has resulted in the development of PRP therapy, which offers potential therapeutic options for a wide range of medical ailments, from musculoskeletal injuries to cosmetic enhancements.

prp centrifuge machine
Figure 1: Platelets Rich Plasma (Fontenot 2012)

Types of PRP centrifuge machine

Platelet-rich plasma (PRP) is a platelet concentration in only a small amount of plasma that can be collected from a blood sample. PRP is frequently utilized in medical therapies to improve tissue repair and healing. The following are some examples of common PRP centrifuges:

Benchtop centrifuges

These are smaller, portable centrifuges designed for use in a laboratory or clinical setting. They are primarily utilized for lesser amounts of blood and are not appropriate for bigger amounts of PRP.

Fixed-angle centrifuge

The most popular type of PRP centrifuge is the fixed-angle centrifuge. They have a set angle of rotation and separate the blood cells with a swinging bucket rotor.

Continuous flow centrifuges

 These devices separate cells by using a continuous flow of blood. They are more efficient and faster than fixed-angle centrifuges, but they are also more expensive.

Automated centrifuges

 These are sophisticated centrifuges with computer controls and automated methods for preparing PRP. They are frequently used in high-volume clinical settings and can be designed to run numerous cycles in a row.

Parts of PRP centrifuge machines


It is the component of the centrifuge that spins the blood sample in order to separate the cells. Different types of rotors, such as fixed-angle rotors or swinging bucket rotors, are employed in different types of centrifuges.


The motor is the component of the centrifuge that drives the centrifugation process by powering the rotor.

Control panel

The control panel of a centrifuge is where the operator sets the required speed and time for the centrifugation. Some control panels may additionally include other functions like a display screen or alarms.

Sample tubes

The containers that store the blood sample throughout the centrifugation process are known as sample tubes. Depending on the exact requirements of the PRP preparation, many types of tubes may be used.


The lid of a centrifuge is the portion that covers the top of the rotor and keeps the blood sample in place during the centrifugation process.

Procedure for operating PRP centrifuge

  •  A small amount of blood is usually collected using a normal venipuncture procedure. The sample should be collected in a sterile container and handled in a sterile manner.
  • To prevent blood clotting, the collected blood sample is usually placed in a tube containing an anticoagulant.
  • The tube is then placed in the centrifuge, and the machine is turned on to rapidly spin the blood. The heavier cells, such as red blood cells, separate from the lighter ones, such as platelets. After the centrifugation process is completed, the PRP is collected from the top layer of the tube.
  • The platelet-rich plasma (PRP) will be transferred to a syringe or other container for use in a medical therapy. To avoid contamination, it is important to use sterile techniques when handling PRP.
  •  The waste material, which includes red blood cells and other cellular debris, should be disposed of in accordance with local legislation.

Applications of PRP centrifuge

  • This platelets rich plasma is frequently utilized for orthopedic surgery to enhance healing and tissue regeneration, such as knee or hip replacements, tendon repair, and ligament restoration.
  • PRP injections are frequently used in sports medicine to treat injuries such as muscular strains, ligament sprains, and tendonitis.
  • Platelet-rich plasma (PRP) is used in a range of cosmetic operations, including face rejuvenation, hair restoration, and scar reduction.
  • PRP is used in a variety of dental procedures, including gum tissue regeneration and tooth implantation.
  • PRP is occasionally utilized in eye surgery, including as corneal transplantation and retinal detachment repair.

Advantages of PRP centrifuge

  • PRP centrifuge devices ensure consistent generation of high-quality PRP, decreasing treatment outcome variability.
  • Because these devices can efficiently separate blood components, PRP preparation is a reasonably quick process.
  •  PRP centrifuge machines allows centrifugation settings to be adjusted to customize PRP concentration based on the specific treatment demands.
  • PRP centrifuge equipment are commonly available in medical facilities, allowing many patients to benefit from PRP therapy.

Disadvantages of PRP centrifuge

  • PRP centrifuge devices can be pricey to buy and maintain, limiting access for smaller medical practices.
  •  Proper operation and maintenance of these equipment needs training and experience, which can be difficult for some facilities to get.
  • PRP quality can still vary depending on machine quality and calibration, operator skill, and blood collecting methodologies.
  • PRP centrifuge machines are primarily utilized for PRP preparation, limiting their versatility when compared to more general-purpose laboratory centrifuges.

Quality control for PRP centrifuge

  • Follow the manufacturer’s recommendations for routine maintenance. All components must be cleaned, lubricated, and inspected.
  • Calibrate the centrifuge machine on a regular basis to ensure that it runs within the parameters indicated. Calibration contributes to the machine’s accuracy and dependability.
  • During each PRP preparation step, ensure that the centrifuge equipment operates at the correct speed; to get results on time. Deviations can have an impact on the quality of PRP.
  • As temperature changes could affect the separation process and the quality of PRP, ensure that the equipment maintains the correct temperature during centrifugation.
  • Maintain records of any centrifuge machine maintenance or calibration.

Key points for PRP centrifuge machines

  • Use a centrifuge that is simple to use, has clear directions, and simple controls.
  • The centrifuge’s speed controls how rapidly the PRP can be prepared. Although faster centrifuges are more efficient, they can be more expensive.
  • Choose a centrifuge that is simple to maintain and provides strong customer service in case you have any problem or require assistance.
  • The centrifuge’s capacity regulates how much blood can be processed at one time. Larger capacity centrifuges may be more efficient in high-volume therapeutic situations, but they are more costly.
  • Consider a centrifuge with safety features like an automated shut-off or an emergency stop button to help prevent accidents and injuries.


The PRP centrifuge machine is a monument to human creativity in the realm of regenerative medicine. Patients and medical experts alike have seen astonishing results from PRP therapy, which harnesses the body’s intrinsic healing ability. As science and technology evolve, the future of PRP therapy shines brightly, promising improved healing, less pain, and increased well-being for many people. So keep an eye on the horizon; the next medical discovery could be spinning in a centrifuge.


Fontenot RL. 2012. Tube centrifugation for processing platelet-rich plasma in the horse. Virginia Tech.

Ujala Shabbir
Ujala Shabbir

Ujala Shabbir is a microbiologist pursuing her MPhil in Microbiology at the University of Veterinary and Animal Sciences in Lahore. Her research is focused on the "preparation of calcium-conjugated FMDV vaccine and its comparative immunogenicity with other vaccine delivery systems".
She completed her Bachelor of Science degree in Applied Microbiology from the same university in 2021 with a CGPA of 3.72/4.
During her undergraduate studies, she took various microbiology-related courses and gained practical experience through internships.

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