Exploring Staphylococcus Aureus | What You Must Need to Know

Table of Contents


Staphylococcus aureus, commonly known as S. aureus, is a significant bacterium that plays a crucial role in both the field of microbiology and its impact on human health. Named after its distinctive clustering shape resembling grapes, this bacterium is a part of the normal flora on the skin and mucous membranes of humans. While many strains of S. aureus are harmless, some can lead to a range of infections, from minor skin conditions to severe life-threatening diseases.

S. aureus exhibits a dual nature, being a commensal organism in the nasal passages and skin, yet capable of causing infections when the balance is disrupted. Understanding the complexities of S. aureus highlights the intricate relationships between microbes and the human body.

Molecular Biology and Genetics

In the realm of molecular biology and genetics, S. aureus has served as a valuable subject for scientific inquiry. Its relatively simple genetic makeup, rapid reproduction rate, and well-understood structure make it an ideal model organism for research. Scientists have utilized S. aureus to explore fundamental concepts in genetics, gene regulation, and mechanisms of antibiotic resistance.

Historical Background

The history of Staphylococcus aureus traces back to its identification by Sir Alexander Ogston in the late 19th century. His groundbreaking work laid the foundation for comprehending the diverse roles that different strains of S. aureus play in the microbial ecosystem. Ogston’s contributions have paved the way for an in-depth exploration of this bacterium’s impact on human health and its significance in scientific research.

Figure 1 Staphylococcus aureus – Source: by Bhavana Kunkalikar

Scientific Classification (Historical)

Historical Taxonomy

Staphylococcus aureus, in its early classification, underwent categorization within the family Staphylococcaceae, a branch of Gram-positive bacteria. Taxonomy heavily relied on morphological characteristics, such as cell arrangement and microscopic features.

Modern Taxonomy

Advancements in taxonomy, particularly molecular techniques, have refined the classification of Staphylococcus aureus:

  • Genus: Staphylococcus
  • Family: Staphylococcaceae
  • Phylum: Firmicutes
  • Class: Bacilli
  • Order: Bacillales
  • Family: Staphylococcaceae
  • Genus: Staphylococcus
  • Species: Staphylococcus aureus

Physiological Characteristics


Staphylococcus aureus exhibits specific physiological traits crucial for its survival:

  • Metabolism: Facultative anaerobe, capable of thriving in both aerobic and anaerobic environments.
  • Energy Production: Utilizes a variety of metabolic pathways to generate energy.

Morphology and Growth

The physiological characteristics of S. aureus extend to its morphological features and growth patterns:

  • Morphology: Spherical cells arranged in grape-like clusters (cocci).
  • Growth: Demonstrates both aerobic and facultative anaerobic growth, allowing colonization in diverse host environments.

Pathogenicity Factors

S. aureus possesses distinct characteristics contributing to its pathogenic potential:

  • Virulence Factors: Produces an arsenal of toxins and enzymes crucial for pathogenesis.
  • Adaptability: Exhibits the ability to evade the host immune response, contributing to its pathogenicity.

Morphology and Structure

Detailed Morphological Description

Staphylococcus aureus, under the microscope, reveals intricate details in its morphology:

1.    Cell Shape and Arrangement

  • Cell Shape: Spherical (cocci).
    • Arrangement: Forms irregular clusters resembling grapes, a characteristic “staphylo” arrangement.
    • Size: Typically ranges from 0.5 to 1.5 micrometres in diameter.

2.    Gram Stain Characteristics

  • Stains purple in the Gram stain due to a thick peptidoglycan layer in the cell wall.
Figure 2 Gram stain of S. aureus – Source: Stepwards

Structural Components

Staphylococcus aureus derives its unique morphology from a complex interplay of structural components:

1.    Cell Wall

Thick peptidoglycan layer

  • Composed of alternating N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) units.
    • Provides structural integrity and shape to the bacterium.

Teichoic acids

  • Embedded in the cell wall.
    • Function in cation binding and cell wall maintenance.

2.    Capsule

  • Some strains may produce a polysaccharide capsule
    • Enhances virulence.
    • Acts as a protective layer, aiding in the evasion of host immune responses.

3.    Cell Membrane

  • Phospholipid bilayer
    • Encases the cytoplasm.
    • Contains integral proteins for transport and permeability.

4.    Flagella

  • Generally non-motile
    • Lacks traditional flagella for movement.
    • Relies on other mechanisms for colonization.

5.    Pili/Fimbriae

  • Hair-like appendages
    • Assist in adhesion to host tissues.
    • Promote biofilm formation.

6.    Cytoplasm

  • Houses genetic material and cellular machinery
    • The Nucleoid region contains circular DNA.
    • Ribosomes for protein synthesis.

7.    Nucleoid

  • An unencapsulated region within the cytoplasm
    • Contains the bacterial chromosome.
    • Essential for genetic replication and transcription.

8.    Plasmids

  • Small, circular DNA molecules
    • May carry accessory genes, including those for antibiotic resistance.
    • Contribute to genetic diversity.

Antigenic Structure

1.    H Antigens (Flagellar Antigens)

  • Staphylococcus aureus is non-motile and lacks traditional flagella.
  • Consequently, H antigens associated with flagellar structures are absent.
  • The absence of H antigens distinguishes S. aureus from motile bacteria.

2.    O Antigens

  • As a Gram-positive bacterium, S. aureus lacks the typical O antigens found in the LPS layer of Gram-negative bacteria.
  • The absence of O antigens contributes to the unique cell wall composition of Gram-positive bacteria.

3.    K Antigens (Capsular Antigen)

  • Some strains of S. aureus express capsular antigens known as K antigens.
  • K antigens contribute to the pathogenicity of the bacterium.
  • Variations in the presence or absence of K antigens influence virulence and immune evasion.

4.    F Antigens (Surface-Associated Antigens)

  • S. aureus may exhibit surface-associated antigens known as F antigens.
  • F antigens are found on the bacterial surface and play a role in adhesion to host tissues.
  • The diversity of F antigens contributes to strain-specific characteristics and interactions with the host environment.

Genetic Characteristics

Genome Overview

Staphylococcus aureus, a bacterium of significant clinical importance, possesses a distinctive genome that plays a crucial role in its biology and pathogenicity.

1.    Genome Size

  1. The genome of S. aureus is approximately 2.8 to 2.9 million base pairs long.
  2. This relatively compact genome contains a diverse array of genes essential for the bacterium’s survival and adaptability.

2.    Circular Chromosome

  1. Like many bacteria, S. aureus harbors a single, circular chromosome.
  2. This chromosomal organization is a fundamental feature of its genetic makeup, housing core genetic information.

3.    Accessory Genetic Elements

  1. In addition to the core chromosome, S. aureus often carries accessory genetic elements such as plasmids.
  2. Plasmids can confer additional traits, including antibiotic resistance and virulence factors.

Genetic Adaptations and Evolution:

S. aureus has displayed remarkable genetic adaptations and evolutionary strategies, contributing to its versatility and ability to thrive in various environments.

1.    Antibiotic Resistance

  1. S. aureus is known for its capacity to adapt rapidly to selective pressures, leading to the emergence of antibiotic-resistant strains.
  2. Genetic elements, including plasmids and mobile genetic elements, facilitate the spread of resistance genes within S. aureus populations.

2.    Virulence Factors

  1. The genome of S. aureus encodes numerous virulence factors, including toxins and adhesion molecules.
  2. Genetic adaptations in virulence factor expression contribute to the bacterium’s ability to establish infections and evade host defenses.

3.    Horizontal Gene Transfer

  1. S. aureus engages in horizontal gene transfer, a process where genetic material is exchanged between individual bacteria.
  2. This mechanism allows for the acquisition of new genetic traits, contributing to the bacterium’s adaptability.

4.    Evolutionary Lineages

  1. S. aureus has diversified into distinct lineages, each characterized by unique genetic signatures.
  2. Evolutionary studies have revealed the emergence of methicillin-resistant Staphylococcus aureus (MRSA) as a notable example of genetic adaptation in response to antibiotic use.

Types of S. aureus

Staphylococcus aureus exists in various forms, each with distinct characteristics and implications:

  1. Methicillin-Resistant Staphylococcus aureus (MRSA): Resistant to methicillin and other beta-lactam antibiotics.
  2. Community-Associated MRSA (CA-MRSA): Acquired outside healthcare settings, often causing skin and soft tissue infections.
  3. Hospital-Associated MRSA (HA-MRSA): Acquired within healthcare settings, posing risks to immunocompromised patients.
  4. Vancomycin-Intermediate Staphylococcus aureus (VISA) and Vancomycin-Resistant Staphylococcus aureus (VRSA): Exhibiting reduced or complete resistance to vancomycin, limiting treatment options.
  5. Colonizing and Infective Strains: S. aureus can be part of normal flora (colonizing) or cause infections, ranging from mild to severe.

Habitat and Ecology

Staphylococcus aureus, primarily found in the natural habitat of the human body, thrives on the skin, particularly in the nasal passages, and may colonize mucous membranes. Its adaptability extends to hospital environments, where hospital-associated strains persist. Globally distributed, S. aureus exhibits diverse ecological roles, acting as both a commensal organism and a pathogen. As a commensal, it peacefully coexists with the host, but certain strains can turn pathogenic, causing infections ranging from localized skin issues to severe systemic diseases. The ecological impact is further influenced by its ability to develop antibiotic resistance, affecting treatment dynamics and transmission patterns.
Pathogenicity and Virulence Factors

Pathogenesis Mechanisms

Staphylococcus aureus, a formidable pathogen, intricately navigates the host’s defenses through sophisticated mechanisms. Initial adhesion to host tissues is facilitated by surface-associated structures, including proteins and adhesins. Once adhered, S. aureus employs biofilm formation—a complex matrix of extracellular polymeric substances—to establish a resilient stronghold, shielding the bacteria from the immune system. Additionally, the bacterium can evade phagocytic cells by inhibiting their action or even surviving within these immune cells.

Table 1 Immune Evasion Strategies of S. aureus through Host Cell Internalization

Internalization by Various Cell TypesS. aureus internalized by fibroblasts, osteoblasts, keratinocytes, and endothelial cellsPotential for systemic spread, antibiotic evasion, and evasion of host immune responses
MSCRAMMs as InvasionsFnbpA and B bind fibronectin, interacting with host cell integrin α5β1Triggers signaling pathways leading to rearrangement of host cell actin cytoskeleton, facilitating bacterial internalization
Chronic and Recurrent InfectionsIntracellular presence observed in chronic infections, e.g., nasal mucosal cells in recurrent rhinosinusitis and tonsillar tissue in recurrent pharyngitisImplication in the chronic and recurrent nature of certain infections
Altered Antibiotic SusceptibilityInternalized S. aureus in mouse osteoblasts showed decreased susceptibility to antibiotics with good intracellular penetration (clindamycin, erythromycin, and rifampin)Suggests a mechanism for antibiotic evasion in an intracellular environment

Different Strains and Associated Diseases

The clinical spectrum of diseases associated with S. aureus is broad and dependent on the strain involved. Community-associated strains commonly cause skin and soft tissue infections, while hospital-associated strains, particularly methicillin-resistant Staphylococcus aureus (MRSA), contribute to severe nosocomial infections, including bloodstream infections and pneumonia. In specific contexts, S. aureus is implicated in gastrointestinal disorders such as gastroenteritis and urinary tract infections (UTIs), showcasing its adaptability to diverse host niches.

Virulence Factors

Table 2 key virulence factors associated with Staphylococcus aureus

Surface AdhesinsProtein A: Binds to the Fc region of immunoglobulins.
Clumping Factor: Promotes adhesion to fibrinogen.
Fibronectin-Binding Proteins: Facilitate attachment.
ToxinsAlpha-Toxin: Forms pores in host cell membranes.
Panton-Valentine Leukocidin (PVL): Targets leukocytes.
Exfoliative Toxins: Cause skin desquamation.
Enterotoxins: Responsible for food poisoning.
EnzymesCoagulase: Initiates blood clotting.
Hyaluronidase: Facilitates tissue invasion.
Lipases and Proteases: Contribute to tissue damage.
CapsuleProtects S. aureus from phagocytosis.
Biofilm FormationEnables bacterial persistence on surfaces.
Immune EvasionProtein A: Interferes with antibody-mediated clearance.
Modulation of Host Immune Response: Various mechanisms.
Figure 3  Virulent factors of S. aureus. – Source: ResearchGate

Clinical Manifestations and Infections

Staphylococcus aureus, a versatile pathogen, elicits a range of clinical manifestations and infections, including

1.    Skin and Soft Tissue Infections

Skin abscesses, cellulitis, and impetigo are common presentations.

Severe cases may lead to necrotizing fasciitis, a potentially life-threatening condition.

2.    Bacteremia and Septicemia

  • Staphylococcus aureus bloodstream infections can result in bacteremia and septicemia.
  • Septic shock may occur, particularly in cases of methicillin-resistant strains (MRSA).

3.    Pneumonia

  • Respiratory infections can progress to pneumonia, marked by fever, cough, and chest pain.
  • Severe cases may involve necrotizing pneumonia, posing significant health risks.

4.    Endocarditis

  • Infection of the heart valves leads to endocarditis, which can result in heart failure.
  • High morbidity and mortality are associated with this invasive infection.

5.    Osteomyelitis

  • Bone infections, especially affecting long bones and joints.
  • Can lead to chronic complications and require prolonged antibiotic treatment.

6.    Toxic Shock Syndrome (TSS)

  • Staphylococcal TSS is characterized by fever, rash, and multi-organ dysfunction.
  • Typically associated with toxin-producing strains of S. aureus.

7.    Device-Associated Infections

  • Infections related to medical devices such as catheters, prosthetic joints, and heart valves.
  • Pose challenges in treatment due to biofilm formation on devices.

8.    Food Poisoning

  • Ingestion of food contaminated with enterotoxins produced by S. aureus.
  • Rapid onset of symptoms, including nausea, vomiting, and diarrhea.

9.    Necrotizing Soft Tissue Infections (NSTIs)

  • Severe infections involving deep layers of skin and soft tissues.
  • Rapid progression and potential for widespread tissue destruction.

10.  Urinary Tract Infections (UTIs)

  • Staphylococcus aureus can cause UTIs, presenting with symptoms like dysuria and frequency.
  • Less common than other pathogens but significant when it occurs.

Laboratory Diagnosis

Diagnostic Tests and Procedures

  • Blood Culture: Blood cultures detect Staphylococcus aureus in the bloodstream, aiding in the diagnosis of bacteremia and septicemia.
  • Wound Swab Culture: Swab cultures from infected sites identify Staphylococcus aureus in skin and soft tissue infections, guiding treatment decisions.
  • Urine Culture: Urine cultures diagnose Staphylococcus aureus in urinary tract infections, facilitating targeted treatment.
  • Sputum Culture:  Respiratory infections, including pneumonia, are diagnosed through sputum cultures to identify Staphylococcus aureus.

Identification Techniques

  • Gram Staining: Gram staining reveals the characteristic appearance of Staphylococcus aureus as a Gram-positive cocci in clusters.
  • Coagulase Test: Positive coagulase tests confirm the presence of Staphylococcus aureus, distinguishing it from other staphylococci.
  • Mannitol Salt Agar (MSA) Culture: MSA cultures differentiate Staphylococcus aureus strains based on their ability to ferment mannitol.
  • DNA-based Methods (PCR): PCR provides rapid and accurate identification of Staphylococcus aureus through amplification of specific DNA sequences.
  • Antibiotic Sensitivity Testing: Assessing susceptibility guides treatment decisions by determining the response of Staphylococcus aureus to antibiotics.
  • Sereny Test (In vivo Assay): The Sereny Test assesses the virulence of Staphylococcus aureus strains, offering insights into potential invasive infections.
  • Cell Culture Test: Cell culture tests observe bacterial behavior and interactions with host cells, enhancing understanding of infection dynamics.

Treatment and Drug Resistance


Management of Staphylococcus aureus infections involves a multifaceted approach. Supportive care is often employed, especially for skin and soft tissue infections, with practices such as wound care and drainage. Mild cases may be resolved with these measures alone. In more severe instances, antibiotic treatment becomes essential. Common choices include vancomycin and daptomycin, particularly in the presence of methicillin-resistant Staphylococcus aureus (MRSA). The selection of antibiotics is influenced by factors like strain type and infection severity.


Healthcare professionals may prescribe antibiotics such as clindamycin, cephalexin, or oxacillin for methicillin-sensitive strains. The choice depends on the specific clinical scenario. Surgical intervention may be necessary for conditions like endocarditis or osteomyelitis, enhancing the effectiveness of antibiotic treatment.


Staphylococcus aureus presents a significant challenge due to the emergence of antibiotic resistance. This growing concern underscores the importance of judicious antibiotic use, adherence to recommended dosages, and robust public health initiatives. Ongoing research and surveillance efforts are critical for monitoring and addressing evolving resistance trends, ensuring effective management of Staphylococcus aureus infections while minimizing the risk of resistance-related complications.

Figure 4 Mannitol salt agar plate with oxacillin showing methicillin-resistant Staphylococcus aureus – Source: ResearchGate

Prevention and Control Strategies

Preventing Staphylococcus aureus infections involves a combination of hygiene practices, vaccination, and infection control measures. Regular handwashing, especially in healthcare settings, helps minimize the risk of transmission. Vaccination against certain strains, such as MRSA, is under exploration. In healthcare facilities, strict adherence to infection control protocols, including proper sanitation and use of personal protective equipment, is crucial. Additionally, antimicrobial stewardship programs promote responsible antibiotic use to curb resistance. Community awareness campaigns on hygiene practices and timely medical attention for skin infections contribute to overall prevention efforts. Combining these strategies is essential to mitigate the spread of Staphylococcus aureus and uphold public health.

Global Impact and Epidemiology

1.    Prevalence

  • Staphylococcus aureus infections have a global presence, affecting diverse populations across continents.
    • Both healthcare-associated and community-acquired infections contribute to the overall prevalence.

2.    Healthcare Burden

  • Staphylococcus aureus is a major contributor to healthcare-associated infections, leading to increased hospitalization rates.
    • The burden extends to conditions such as bloodstream infections, pneumonia, and surgical site infections.

3.    Antibiotic Resistance

  • The global rise in antibiotic resistance, especially in methicillin-resistant strains (MRSA), amplifies the challenges in treating Staphylococcus aureus infections.
    • Resistance trends vary regionally, requiring continuous surveillance and adaptation of treatment strategies.

4.    Impact on Public Health

  • Staphylococcus aureus infections pose a substantial public health threat due to their potential for severe and invasive manifestations.
    • The associated morbidity and mortality, particularly in vulnerable populations, contribute to the overall impact.

5.    Economic Consequences

  • The economic burden of Staphylococcus aureus infections is significant, encompassing healthcare costs, productivity losses, and expenses related to antibiotic resistance management.

6.    Epidemiological Trends

  • Epidemiological patterns of Staphylococcus aureus infections exhibit variability, influenced by factors such as geographical location, healthcare infrastructure, and socio-economic conditions.
    • Continuous surveillance is crucial to monitor and understand evolving trends, informing preventive and control strategies.

7.    Global Initiatives

  • International efforts focus on collaborative research, surveillance, and sharing of best practices to address the global impact of Staphylococcus aureus.
    • Initiatives promoting antibiotic stewardship and infection prevention contribute to the broader goal of mitigating the impact on a worldwide scale.

Environmental and Industrial Applications

1.    Bioremediation

  • Staphylococcus aureus strains have been explored for their potential in bioremediation processes, aiding in the cleanup of environmental pollutants.
    • The bacteria’s metabolic activities can contribute to the degradation of various contaminants.

2.    Enzyme Production

  • Staphylococcus aureus strains have been studied for their ability to produce enzymes with industrial applications.
    • Enzymes such as lipases and proteases have relevance in processes like food production and detergent manufacturing.

3.    Biotechnological Research

  • Staphylococcus aureus serves as a model organism in biotechnological research.
    • Its genetic tractability and ability to produce various biomolecules contribute to advancements in genetic engineering and bioprocessing.

4.    Fermentation Processes

  • Some strains of Staphylococcus aureus are employed in industrial fermentation processes.
    • They play a role in the production of certain fermented foods and beverages, contributing to their flavor and texture.

5.    Vaccine Development

  • Research involving Staphylococcus aureus contributes to the development of vaccines with potential applications in both medical and industrial settings.
    • Vaccines targeting specific strains, especially those associated with antibiotic resistance, are of interest.

6.    Quality Control in Pharmaceuticals

  • Staphylococcus aureus is used in quality control processes within the pharmaceutical industry.
    • It serves as a test organism to assess the efficacy of antimicrobial agents and validate sterilization procedures.

7.    Biopharmaceutical Production

  • Staphylococcus aureus strains engineered for specific purposes are explored for biopharmaceutical production.
    • The bacteria’s ability to express and produce proteins can be harnessed for therapeutic applications.


  • Tong, S. Y., Davis, J. S., Eichenberger, E., Holland, T. L., & Fowler Jr, V. G. (2015). Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clinical microbiology reviews, 28(3), 603-661.
  • Gnanamani, A., Hariharan, P., & Paul-Satyaseela, M. (2017). Staphylococcus aureus: Overview of bacteriology, clinical diseases, epidemiology, antibiotic resistance and therapeutic approach. Frontiers in Staphylococcus aureus, 4(28), 10-5772.
  • Foster, T. J. (2002). Staphylococcus aureus. Molecular Medical Microbiology, 839-888.
  • Lowy, F. D. (1998). Staphylococcus aureus infections. New England journal of medicine, 339(8), 520-532.
  • Pillai, M., R, L., & Sarkar, G. (2012). Detection of Methicillin Resistance in Staphylococcus Aureus by Polymerase Chain Reaction and Conventional Methods: A Comparative Study. Journal of laboratory physicians, 4, 83-88. https://doi.org/10.4103/0974-2727.105587
  • Cheung, G. Y. C., Bae, J. S., & Otto, M. (2021). Pathogenicity and virulence of Staphylococcus aureus. Virulence, 12(1), 547-569. https://doi.org/10.1080/21505594.2021.1878688
Bareera Aatif
Bareera Aatif

Bareera Aatif is currently pursuing a Bachelor of Science in Microbiology at the University of Central Punjab, Lahore. With a strong practical foundation gained from a six-month internship at Ferozsons Pharmaceutical Laboratories - BF Biosciences and a one-month experience at PepsiCo International, she combines academic rigor with real-world expertise. Beyond academics, Bareera actively contributes to university societies as an event manager, content writer, and graphics designer, showcasing her versatile skill set. Her commitment to microbiological research and dedication to continuous learning positions her as a promising professional poised for impactful contributions in the field.

Articles: 6

Newsletter Updates

Enter your email address below and subscribe to our newsletter

Leave a Reply

Your email address will not be published. Required fields are marked *